Period: 11.2017 – 4.2019

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Project team:

Prof. Dr. Beatrix Süß | Dr. Florian Groher | Jeannine Schneider, M. Sc.
FB 10, Synthetische Genetische Schaltkreise

Prof. Dr. Edgar Dörsam | Dr.-Ing Dieter Spiehl | Jacqueline Balles, M. Sc.
FB 16, Institut für Druckmaschinen und Druckverfahren

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In this project, printed biosensors for the detection of antibiotic residues in food were developed in cooperation between biology and mechanical engineering. The underlying idea is that antibiotics such as ciprofloxacin (CFX) and tetracycline (Tc), which are present as residues in food, have intrinsic fluorescence which is reduced (quenched) by binding to a suitable RNA aptamer. The Biology Department has developed a detector system that synthesizes and analyzes aptamers and makes them available for compression tests. The Mechanical Engineering department established the printing process, i.e. a suitable printing process, ink formulation, substrate and design. Preliminary work led to first publications at a conference [1] and in a journal [2], both for printing technology. Based on the results, a CFX aptamer was established and a biosensor was printed by inkjet printing, which was also published [3]. In further cooperation, a universal detector system will be developed, which can be visually read by a color change of gold nanoparticles. A DFG proposal is currently being finalised.

TUbiblio:

DNA and DNA staining as a test system for the development of an aptamer-based biosensor for sensing of antibiotics

A test system for the printing of functional nucleic acids onto different carriers and verification of its functionality by DNA dyes

Characterization and Inkjet Printing of an RNA Aptamer for Paper-Based Biosensing of Ciprofloxacin.

Druckbare Biosensoren – Ein RNA basiertes Nachweissystem auf Papier. (Poster)